Keeping Your Production Sterile: MFT Solutions for Every Lab

Maintaining sterility in pharmaceutical manufacturing is never simple. While heat-based methods such as steam or dry sterilization are effective, they are not suitable for every product, as certain formulations could degrade or lose their therapeutic value.

To address this, the industry relies on Media Fill Tests (MFTs). By replacing the product with a sterile nutrient medium, this simulation recreates the aseptic process under real conditions, providing solid evidence that the production line can consistently deliver sterile products.

The Media Fill Test (MFT) is included in key international standards such as the European (EP), United States (USP), and Japanese (JP) pharmacopoeias. Its purpose is to replicate the aseptic production process as closely as possible: from setting up the equipment, through the sterile filtration and transfer of product volumes, to the final filling of containers. Each of these steps is reproduced with a nutrient medium instead of the actual product, providing solid proof that sterility is maintained throughout the entire workflow.

For an MFT to be reliable, the choice of culture medium is critical. It must support the growth of a wide range of microorganisms while offering clarity and consistency throughout the simulation. Bearing this in mind, at Alpha Biosciences we are in a constant process of development and optimization of our products to meet the needs of the market. This is why we offer several options to offer a service tailored to the needs of our customers:

• Trypto Soy Broth (T20-110): A classic and trusted choice, TSB supports a wide range of bacteria and works reliably in all MFT applications.
• Fluid Thioglycollate Medium (F06-101): Ideal for strict anaerobes, this medium creates a precise oxygen gradient thanks to its reducing agent, allowing accurate detection and differentiation of oxygen requirements.
• Trypto Soy Broth, Animal Free (T20-137): A versatile, animal-free alternative that maintains the richness and reliability of classic TSB, making it perfect for labs seeking sustainable, animal-free solutions.

We don’t just provide standard culture media; we can develop custom solutions tailored to your unique needs. In addition, we supply high-quality media for the pharmaceutical industry and other laboratories, ensuring reliable results every time.

Contact us today to explore all the possibilities and discover how we can support your lab.