Columbia Broth is used for the cultivation of fastidious and non-fastidious organisms. Eller et al. First described Columbia broth. It was designed to produce rapid and luxuriant growth of a range of organisms due to its high nutrient content. It contains meat peptone, casein peptone and yeast extract, all of which are highly nutritious components. The meat peptone contains sufficient sulfonamide antagonists to prevent sulfonamide inhibition of growth. Columbia Broth, in the presence of CO2 and supplemented with SPS is an excellent blood culture medium.