DNase Test Agar is used to detect deoxyribonuclease activity of bacteria and fungi, particularly for the identification of Staphylococci. Tryptose provides nitrogenous sources, carbohydrates, minerals, and trace elements. Toluidine Blue allows for the detection of DNase activity on DNA, resulting in the production of a bright pink reaction due to the metachromatic property of this indicator. Some strains of Staphylococci may be inhibited on DNase Test Agar due to toluidine blueSodium Chloride maintains the osmotic balance and Agar is the gelifying agent.
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