TROBLESHOOTING DEHYDRATED MEDIA ISSUES
Incorrect pH
· Improperly washed glassware
· Impure water
· Incorrect weigh out
· Incomplete mixing
· Overheating
· pH measured at incorrect temperature
· Incorrect storage of media
Atypical Precipitate or Abnormal Color
· Improperly washed glassware
· Impure water
· Incorrect weigh out
· Incomplete mixing
· Overheating
Trace Substaces or Incomplete Solubility
· Burning or scorching of media
· Improperly washed glassware
· Impure water
Loss of Geling Properties
· Incorrect weigh out
· Incomplete mixing
· Overheating
· Dilution by too large of inoculum
· Hydrolysis of agar due to pH shift
Trobleshooting Dehydrated Media Issues
Incorrect pH
- Improperly washed glassware
- Impure water
- Incorrect weigh out
- Incomplete mixing
- Overheating
- pH measured at incorrect temperature
- Incorrect storage of media
Atypical Precipitate or Abnormal Color
- Improperly washed glassware
- Impure water
- Incorrect weigh out
- Incomplete mixing
- Overheating
Trace Substaces or Incomplete Solubility
- Burning or scorching of media
- Improperly washed glassware
- Impure water
Loss of Geling Properties
- Incorrect weigh out
- Incomplete mixing
- Overheating
- Dilution by too large of inoculum
- Hydrolysis of agar due to pH shift
