SFP Agar Base, with the addition of selective supplements and enrichments, is used for the identification and quantification of Clostridium perfringens in food samples. Tryptose, Papaic Digest of Soybean Meal, and Yeast Extract supply nitrogenous compounds, carbon, sulphur, B-complex vitamins, and minerals necessary for the growth of Clostridia. Sodium Bisulphite and Ferric Ammonium Citrate are the indicators of sulphite reduction by C. perfringens, which produces black colonies. Kanamycin and Polymyxin B inhibit competitive bacteria and thus allow a better recovery of vegetative cells and spores of C. perfringens. Some strains of C. perfringens may form an opaque zone around the colony due to their lecithinase activity. Lecithinase-positive facultative anaerobes may grow on SFP Agar making the plates completely opaque and thus may mask the egg yolk reaction of C. perfringens. Organisms other than C. perfringens may produce black colonies. Therefore, presumptive C. perfringens colonies need to be further confirmed by motility test, nitrate reduction, and gelatin liquefaction. Agar is the gelifying agent.
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