SIM Medium
SIM Medium is a semisolid medium used for the determination of indole formation, sulfide production, and motility of the Enterobacteriaceae, especially Salmonella and Shigella. Ferrous Ammonium Citrate and Sodium Thiosulfate are used to detect hydrogen sulfide production by Salmonella. H2S gas reacts with Ferrous Ammonium Citrate to produce ferrous sulfide, a black precipitate. Casein Peptone contains tryptophan, which is converted to indole by Shigella. Indole is detected after incubation by the addition of Kovac&rsquo