Tryptose Phosphate Broth
Tryptose Phosphate Broth is a liquid medium recommended for the cultivation of fastidious microorganisms. Enzymatic Digest of Casein, Soy Peptone, and Yeast Extract supply most of the nutrients for bacterial growth (amino acids, peptides, carbohydrates, vitamins, and minerals). Dextrose is the main carbohydrate source. Sodium Chloride provides essential electrolytes and maintains osmotic equilibrium. Disodium Phosphate acts as pH buffer. The addition of 0.1 – 0.2% Agar concentration can aid in the recovery of anaerobes.