Triple Sugar Iron Agar is used as a differential medium for the presumptive identification of enteric bacteria. The medium contains no inhibitors and most organisms, including Gram-positive organisms and yeasts, can grow on it. Phenol Red indicates fermentation of the carbohydrates Dextrose, Sucrose and Lactose when it shifts to yellow due to acidification of the medium. Ferric Ammonium Citrate indicates hydrogen disulfide production through the reduction of Sodium Thiosulfate. Upon reaction with this gas, it causes a blackening in the middle and upper portions of the slant.
Buffered Peptone Water is used for pre-enriching damaged Salmonella spp. from various food sources. Edel and Kampelmacher noted that food preservation techniques such as heat, desiccation, high...
Rapport-Vassiliadis R10 Broth is used for the selective enrichment of Salmonella spp. from meat products, feces, and water polluted by sewage. The medium was formulated by Rapport and modified by...
XLD Agar is used for isolating and differentiation of Gram-negative enteric bacteria. In this medium, Yeast Extract provides the nitrogenous sources and carbohydrates required for bacterial growth...
Nutrient Agar is a solid medium used for the non-selective growth of a wide variety of non-fastidious organisms. Pancreatic Digest of Gelatin and Beef Extract provide amino acids, peptides,...
