Brilliant Green Agar
Brilliant Green Agar is a selective medium used for the isolation of Salmonella spp. other than S. typhi. Brilliant Green Agar was first described by Kristensen in 1925 and later modified by Kauffman in 1935. Brilliant Green inhibits Gram-positive and most Gram-negative bacteria, while Phenol Red serves as a pH indicator by changing the color of the medium to yellow when fermentation of Sucrose and Lactose occurs. Proteose Peptone No. 3 and Yeast Extract provide essential nutrients for bacterial growth, while Sodium Chloride supplies electrolytes and maintains osmotic equilibrium. Agar is the gelifying agent.