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Trobleshooting Dehydrated Media Issues
Incorrect pH
- Improperly washed glassware
- Impure water
- Incorrect weigh out
- Incomplete mixing
- Overheating
- pH measured at incorrect temperature
- Incorrect storage of media
Atypical Precipitate or Abnormal Color
- Improperly washed glassware
- Impure water
- Incorrect weigh out
- Incomplete mixing
- Overheating
Trace Substaces or Incomplete Solubility
- Burning or scorching of media
- Improperly washed glassware
- Impure water
Loss of Geling Properties
- Incorrect weigh out
- Incomplete mixing
- Overheating
- Dilution by too large of inoculum
- Hydrolysis of agar due to pH shift